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Microsatellite pre-screening identified 13 (27%) of the Escarpia loci were homologous and polymorphic in S. Loci found to be both polymorphic and of high quality were used to test for significant population structuring in S. Forty-eight microsatellite loci that were originally developed for Escarpia laminata (18 loci) and Escarpia southwardae (30 loci) were tested to determine if they were homologous and polymorphic in S. jonesi individuals were collected from eight seep sites across the upper Louisiana slope (<1,000 m) in the GoM. Additionally, we determine if cross-amplified loci can reliably uncover the same signatures of high gene flow seen in a previous investigation of S. could be amplified in the GoM seep tubeworm, Seepiophila jonesi. In the present study, we investigate the utility of microsatellites to be cross-amplified within the escarpiid clade of seep vestimentiferans, by determining if loci originally developed for Escarpia spp. As such, researchers have applied microsatellite markers as a measure for documenting the transport of vestimentiferan individuals. Several studies have investigated tubeworm population structure however, much is still unknown about larval dispersal patterns at Gulf of Mexico (GoM) seeps. Vestimentiferan tubeworms are some of the most recognizable fauna found at deep-sea cold seeps, isolated environments where hydrocarbon rich fluids fuel biological communities. Investigation of population structure in Gulf of Mexico Seepiophila jonesi (Polychaeta, Siboglinidae) using cross-amplified microsatellite loci. Cite this article Huang C, Schaeffer SW, Fisher CR, Cowart DA. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. Licence This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. 2 Department of Animal Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United States DOI 10.7717/peerj.2366 Published Accepted Received Academic Editor Karen Osborn Subject Areas Conservation Biology, Ecology, Genetics, Genomics, Marine Biology Keywords deep sea, hydrocarbon seep, vestimentiferan tubeworm, siboglinid, microsatellite, population structure, gene flow Copyright В© 2016 Huang et al.

Genodive unable to read population number